PhD Dissertation at the University of Basrah Investigates the Evaluation of Apple Seed Emulsion on Normal and Cancerous Cell Lines

A PhD dissertation at the College of Veterinary Medicine, University of Basrah, explored the cytotoxic and therapeutic potential of a nano-emulsion derived from apple seed oil on both normal and cancerous cell lines.

The dissertation, presented by PhD candidate Ruqayya Kadhim Jawad, aimed to assess the effectiveness of the apple seed oil nano-emulsion on the normal cell line (Vero-Salm) and the human skin cancer cell line (A431), through in vitro experiments as well as in vivo trials using laboratory animals.

The study involved inducing dermatitis in adult male rats using a chemical irritant, followed by treatment with the nano-emulsion through two methods: topical application to the skin and intraperitoneal injection. The therapeutic effects were then evaluated based on histopathological changes in the skin and other key organs.

Additionally, the research included immunohistochemical assays to detect immune-related protein markers and to determine the emulsion's impact on the pathological changes induced by the chemical agent.

The dissertation concluded that the apple seed oil nano-emulsion exhibited significant cytotoxic activity against A431 cancer cells. It also demonstrated a notable anti-inflammatory effect, gradually restoring tissue structures to their normal state and reducing the expression of immune-related proteins in histological samples.

Prepared by: The Division of Media and Governmental Communication

College of Veterinary Medicine – University of Basrah